Technical Data
A0225-01A
AcCoA Carboxylase 1 (ACC-1, Acetyl Coenzyme A Carboxylase 1)
Description:
In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA from acetyl-CoA by Acetyl-CoA carboxylase is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases and regulated by ACC-2. Activities of ACC-1 and 2 are regulated by their phosphorylation by AMPK. Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is a cytosolic enzyme, enriched in liver, adipose and lactating mammary tissues. ACC1 catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Two variants of ACC-1 have been described: one with 8 additional aa commencing at Pro-1196, and the other which is 59aa shorter than the predominant fat and liver isoform exist in mammals. The presence of 8 additional aa inhibits the in vitro phosphorylation of the Ser1200 by camp-dependent kinase. The two ACC1 isoforms are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:100,000. Using 50-100ng of control peptide/well (A0225-01: AcCoA Carboxylase 1, Human, Control Peptide (ACC-1, Acetyl Coenzyme A Carboxylase 1)).
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to a 15aa sequence near the N-terminus of human AcCoA Carboxylase 1 (KLH). Species Sequence Homology: rat, mouse, bovine, porcine, sheep: 100%; chicken, 86%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes human Acetyl-CoA Carboxylase-1 (ACC-1). No significant sequence homology is seen with ACC-2 or any other protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Munday, M.R., et al., Eur. J. Biochem. 175: 331 (1988). 2. Abu-Elheiga, et al., PNAS 97: 1444 (2000). 3. Lee, et al., J. Biol. Chem. 276: 2576 (2001). 4. Abu-Elheiga, et al., J. Biol. Chem. 272: 10,699 (1997). 5. Hoppel, et al., ABB 392: 321 (2001). 6. Fraser & Zammit, Biochem. J. 329: 225 (1998). 7. Stapleton, et al., J. Biol. Chem. 271: 611 (1996). 8. Mitchelhill, et al., J. Biol. Chem. 272: 24,475 (1997).