Technical Data
AcCoA Carboxylase 1 (ACC-1, Acetyl Coenzyme A Carboxylase 1)
In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA from acetyl-CoA by Acetyl-CoA carboxylase is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases and regulated by ACC-2. Activities of ACC-1 and 2 are regulated by their phosphorylation by AMPK. Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is a cytosolic enzyme, enriched in liver, adipose and lactating mammary tissues. ACC1 catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Two variants of ACC-1 have been described: one with 8 additional aa commencing at Pro-1196, and the other which is 59aa shorter than the predominant fat and liver isoform exist in mammals. The presence of 8 additional aa inhibits the in vitro phosphorylation of the Ser1200 by camp-dependent kinase. The two ACC1 isoforms are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:100,000. Using 50-100ng of control peptide/well (A0225-01: AcCoA Carboxylase 1, Human, Control Peptide (ACC-1, Acetyl Coenzyme A Carboxylase 1)).
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to a 15aa sequence near the N-terminus of human AcCoA Carboxylase 1 (KLH). Species Sequence Homology: rat, mouse, bovine, porcine, sheep: 100%; chicken, 86%.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Recognizes human Acetyl-CoA Carboxylase-1 (ACC-1). No significant sequence homology is seen with ACC-2 or any other protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Munday, M.R., et al., Eur. J. Biochem. 175: 331 (1988). 2. Abu-Elheiga, et al., PNAS 97: 1444 (2000). 3. Lee, et al., J. Biol. Chem. 276: 2576 (2001). 4. Abu-Elheiga, et al., J. Biol. Chem. 272: 10,699 (1997). 5. Hoppel, et al., ABB 392: 321 (2001). 6. Fraser & Zammit, Biochem. J. 329: 225 (1998). 7. Stapleton, et al., J. Biol. Chem. 271: 611 (1996). 8. Mitchelhill, et al., J. Biol. Chem. 272: 24,475 (1997).