Technical Data
A0225-03
AcCoA Carboxylase 2 (ACC-2, Acetyl Coenzyme A Carboxylase 2)
Description:
In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene- expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC-2 (rat 2456aa, human 2483aa, ~280kD, chromosome 12q24.1), also known as ACC-beta, is predominantly present in heart and skeletal muscle and to a lesser extent in liver. An additional ACC-2 isoform (270kD) is present in liver. In contrast to ACC-1, which is cytosolic and catalyzes only fatty acid synthesis, ACC-2 co-localizes with CPT-1 in the 'contact sites' of the mitochondrial membranes and regulates mitochondrial fatty acid oxidation as well by inhibiting CPT-1 by its product malonyl-CoA. ACC-2 contains an unique 114aa long N-terminus peptide, accounting in part, for its regulatory role in fatty acid oxidation. ACC2 deficient mice accumulate 10-30 fold less malonyl-CoA in heart and muscle and show 50% less fat in the adipose tissue.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide:
A0225-03B: AcCoA Carboxylase 2, Control Peptide, Rat (ACC-2, Acetyl Coenzyme A Carboxylase 2)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide consisting of 20aa sequence mapping near the N-terminus of rat ACC-2.
Purity:
Serum
Form
Supplied as a liquid, neat serum, 0.1% sodium azide, 40% glycerol.
Specificity:
Recognizes AcCoA Carboxylase 2. No significant sequence homology is seen with ACC-1 or any other protein. Species Sequence Homology: Mouse: 75%; human: 60%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Abe, K., et al., BBA 1398: 347-352 (1998). 2. Widemer, J., et al., Biochem. J. 316: 915-922 (1997). 3. Abu-Elheiga, et al., J. Biol. Chem. 272: 10,699 (1997). 4. Abu-Elheiga, et al., PNAS 97: 1444 (2000). 5. Lee, et al., J. Biol. Chem. 276: 2576 (2001). 6. Hoppel, et al., ABB 392: 321 (2001). 7. Fraser & Zammit, Biochem. J. 329: 225 (1998). 8.Stapleton, et al., J. Biol. Chem. 271: 611 (1996). 9.Mitchelhill, et al., J. Biol. Chem. 272: 24,475 (1997).