Technical Data
Acetylcholine, Conjugated
Suitable for use in ELISA, Immunocytochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Crossreactivity Data:
Using a conjugate Choline-GA-PC, antibody specificity was performed with an ELISA test by competion experiments with the following compounds.

Compound Crossreactivity ratio (a)
Choline-PC    1
Phosphatidylcholine (free) >1:10,000
Choline (free) >1:10,000
Acetylcholine (free) >1:10,000

(a): Choline-PC concentration/unconjugated or conjugated analogs concentration at half displacement.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20°CBlue IceRabbit
~1mg/ml (after reconstitution)
Synthetic choline conjugated to protein carrier (PC).
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH20.
Recognizes protein conjugated Acetylcholine. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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