Technical Data
Acetylcholine, Conjugated
Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:5000.
Optimal dilutions to be determined by the researcher.

Crossreactivity Data:
Using a conjugate Choline-GA-Protein, antibodies specificity was performed with an ELISA test by competion experiments with the following compounds.

Compound Crossreactivity ratio (a)
Choline-GA-PL 1
Phosphatidylcholine > 1:10,000
Choline > 1:10,000
Acetylcholine > 1:10,000
Choline-allyl alcohol-G-BSA > 1:1.6

(a): Choline-GA-BSA concentration/unconjugated or conjugated analogs concentration at half displacement.
GA= Glutaric anhydride
PL= Poly lysine
BSA= Bovine Serum Albumin

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug4C (-20C Glycerol)Blue IceRabbit
Choline-glutaric anhydride (GA)-BSA
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Supplied as a liquid in PBS, pH 7.2, before the addition of glycerol to 40%. No preservative added.
Recognizes protein conjugated Acetylcholine. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. GEFFARD, M., VIEILLEMARINGE, J., HEINRICH-ROCK, A.M., DURIS, P.: Anti-acetylcholine antibodies and first immunocytochemical application in insect brain. Neurosci. Lett. 57: 1-6 (1985). 2. GEFFARD, M., McRAE-DEGUEURCE, A., SOUAN, M.L.: Immunocytochemical detection of acetylcholine in the rat central nervous system. Science 229: 77-79 (1985). 3. McRAE-DEGUEURCE, A., GEFFARD, M.: One perfusion mixture for immunocytochemical detection of noradrenaline, dopamine, serotonin and acetylcholine in the same rat brain. Brain Res. 376: 217-219 (1986). 4. WANG-BENNETT, L.T., SOUAN, M.L., GLANTZ, R.M.: Immunocytochemical studies of the distribution of acetylcholine in the crayfish. Brain J. of Comparative Neurol. 273: 330-343 (1988).