Technical Data
Acetylcholine Assay Kit, BioAssay™
Kits and Assays Storage: -20°CShipping: Dry Ice
Acetylcholine Assay Kit provides a simple, direct and high throughput assay for measuring acetylcholine in biological samples.

Principle of the Assay:
In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585nm) is directly proportional to the acetylcholine concentration in the sample.

Assays: Determination of acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue and cell culture.
Drug Discovery/Pharmacology: Investigation of effects of drugs on acetylcholine metabolism.

Sample Size: 20ul

Linear Detection Range: 100-200uM (fluorometric assay); 0.4-10uM (colorimetric assay)

Kit Components:
A0526-32A1: ACHE Enzyme, 1x120ul
A0526-32A2: Enzyme Mix, 1x120ul
A0526-32A3: Acetylcholine Standard (2mM), 1x400ul
A0526-32A4: Dye Reagent, 1x120ul
A0526-32A5: Assay Buffer, 1x10ml

Storage and Stability:
Store all components at -20°C. Kit is stable for up to 3 months upon receipt.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Vizi, E.S. et al (1985). A simple and sensitive method of acetylcholine identification and assay. Bioassay combined with minicolumn gel filtration or high-performance liquid chromatography. J Pharmacol Methods. 13:201-211. 2. Gilberstadt, M.L. and Russell, J.A. (1984). Determination of picomole quantities of acetylcholine and acetylcholine in physiologic salt solutions. Anal Biochem. 138:78-85. 3. Israel, M. and Lesbats, B. (1982). Application to mammalian tissues of the chemiluminescent method for detecting acetylcholine. J Neurochem. 39:248-250.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.