Technical Data
A0526-32A
Acetylcholine Assay Kit, BioAssay™
1 Kit
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Technical Data |
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A0526-32A |
Acetylcholine Assay Kit, BioAssay™ |
1 Kit |
| Kits and Assays | Storage: -20°CShipping: Dry Ice |
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The United States Biological Acetylcholine Assay Kit provides a simple, direct and high throughput assay for measuring acetylcholine in biological samples. Principle of the Assay: In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585nm) is directly proportional to the acetylcholine concentration in the sample. Applications: Assays: Determination of acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue and cell culture. Drug Discovery/Pharmacology: Investigation of effects of drugs on acetylcholine metabolism. Sample Size: 20ul Linear Detection Range: 100-200uM (fluorimetric assay); 0.4-10uM (colorimetric assay) Kit Components: A0526-32A1: ACHE Enzyme, 1x120ul A0526-32A2: Enzyme Mix, 1x120ul A0526-32A3: Acetylcholine Standard (2mM), 1x400ul A0526-32A4: Dye Reagent, 1x120ul A0526-32A5: Assay Buffer, 1x10ml Storage and Stability: Store all components at -20°C. Kit is stable for up to 3 months upon receipt. CAS Number: N/A |
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Vizi, E.S. et al (1985). A simple and sensitive method of acetylcholine identification and assay. Bioassay combined with minicolumn gel filtration or high-performance liquid chromatography. J Pharmacol Methods. 13:201-211. 2. Gilberstadt, M.L. and Russell, J.A. (1984). Determination of picomole quantities of acetylcholine and acetylcholine in physiologic salt solutions. Anal Biochem. 138:78-85. 3. Israel, M. and Lesbats, B. (1982). Application to mammalian tissues of the chemiluminescent method for detecting acetylcholine. J Neurochem. 39:248-250.
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