Technical Data
A0526-42F
Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor, ARACHE, N-ACHE-YT)
Description:
Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Applications:
Suitable for use in Immunohistochemistry and Immunoprecipitation. Not recommended for Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:1000
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b6D90Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatMouse
Concentration:
Not determined
Immunogen:
Purified rat brain AChE (acetylcholinesterase)
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Specificity:
Recognizes rat Acetylcholinesterase YT blood group. Reacts with both the soluble and membrane-associated AChE. Immunohistochemical staining of AChE in rat brain results in staining of nerve fibers and terminals. Species crossreactivity: guinea pig, rabbit, feline. Does not cross-react with BChE (butyrylcholinesterase).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. S. Brimijoin and V.A. Lennon; J. Neural Transmission Suppl. 34, 139 (1991) 2. Monoclonal antibodies to rat brain acetylcholinesterase: comparative affinity for soluble and membrane-associated enzyme and for enzyme from different vertebrate species: Z. Rakonczay and S. Brimijoin; Neurochemistry 46, 280 (1986)