Acetylcholinesterase (AChE, EC.22.214.171.124.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320kD, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170kD.
Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects.
Suitable for use in ELISA (1), Western Blot. Other applications not tested.
Western Blot: 1:75 (1)
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the eruthrocyte enzyme. Coupled to PPD with glutaraldehyde.|
|Purified by Protein A/G affinity chromatography from culture supernatant. |
|Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.|
|Specific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and erythrocyte AChE. Weak cross-reactivity with Torpedo marmorata AChE but none with AChE from electric eel or human BtChE.Epitope: C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE) (1). Can be used in ELISA on amniotic fluid for the diagnosis of neural tube defects. Well suited as catching antibody (on an anti-mouse IgG coat) in enzyme antigen immunoassay (EAIA), where the antigen (AChE) is captured and used directly as substrate for acetylthiocholiniodide (Ellmann’s reaction) (1,2). In Western blotting and dot blotting, this antibody reacts with native and denatured human and bovine, detergent soluble and salt-soluble AChE. No cross-reactivity is seen with erythrocyte AChE.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Boschetti N, Brodbeck U, Jensen SP, Koch C, Norgaard-Pedersen B (1996) Monoclonal antibodies against a C-terminal peptide of human brain acetylcholinesterase distinguish between erythrocyte and brain acetylcholinestrases. Clin Chem 42:19-23. 2. Rasmussen AG, Arends J, Larsen SO (1989) Evaluation and quality control of a monoclonal antibody based enzyme antigen immunoassay of acetylcholinesterase in amniotic fluid. Scand J Clin Lab Invest 49:503-11. 3. Aziz-Aloya R, Sternfeld M, Soreq H (1993) Promoter elements and alternative splicing in the human ACHE gene. Prog Brain Res 98:147-153. 4. Sorensen K, Gentinetta R, Brodbeck U (1982) An amphiphile-dependent form of human brain caudate nucleus acetylcholinesterase: purification and properties. J Neurochem 39:1050-1060.|