Technical Data
A0526-45
Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor, ARACHE, N-ACHE-YT)
Description:
Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Applications:
Suitable for Imminoblot (Western or Dot Blot), ELISA, Immunoprecipitation, Conjugation and most immunological methods. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:5000; 1ug of Acetyl Cholinesterase was used in a standard sandwich ELISA with rabbit anti-goat (HRP)and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-28K: IgG, H&L (HRP) Pab RbxGt
I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
10mg4C (-20C Glycerol)Blue IceBovineGoat
Concentration:
~10mg/ml
Immunogen:
Acetyl Cholinesterase (Bovine Erythrocytes)
Purity:
Purified by delipidation, salt fractionation and ion exchange chromatography.
Form
Supplied as liquid in PBS, 0.1% sodium azide, 40% glycerol.
Specificity:
Recognizes bovine Acetyl Cholinesterase. Assay by IEP resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified Acetyl Cholinesterase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.