Technical Data
A0560-06
Acid Phosphatase (Potato) (Biotin)
Description:
Applications:
Suitable for Western Blot and ELISA.. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000-1:32,000.
Assayed against 1ug Acid Phosphatase in standard capture ELISA using Streptavidin (HRP) (Catalog No: S7973-97A) and ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO (Catalog No: A0030).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Also available:
A0560: Acid Phosphatase (Potato) Pab Rb x (antiserum)
A0560-05: Acid Phosphatase (Potato) Pab Rb x (Highly purified)
A0560-06: Acid Phosphatase (Potato) (Biotin) Pab Rb x
A0560-07: Acid Phosphatase (Potato) (HRP) Pab Rb x
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceRabbit
Concentration:
~10mg/ml
Immunogen:
Acid Phosphatase (Potato).
Purity:
Puri ed by delipidation, fractionation and ion exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glcyerol. No stabilizing proteins added. Labeled with Biotinyl N-hydroxysuccinimide ester (BNOHSA, Biotin). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Specificity:
Recognizes Acid Phosphatase. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as puri ed and partially puri ed Acid Phosphatase (Potato).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.