Technical Data
A0560-21A
Acid Phosphatase 1b (ACP1b, ACP1, Adipocyte Acid Phosphotase, HAAP, Low Molecular Weight Cytosolic Acid Phosphatase, Low Molecular Weight Phosphotyrosine Protein Phosphotase, LMW-PTP, LMW-PTPase, Red Cell Acid Phosphotase 1)
Description:
ACP1 (E.C 3.1.3.2) is a unique member of Phosphotyrosine Protein phosphotase family of proteins. It is ubiquitously expressed acid phosphotase functioning as an acid phosphotase and a protein tyrosine phosphotase. ACP1 has a cysteine-based catalytic mechanism and hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate. Reports suggest a functional role of ACP1 in TCR signaling and control of cell-cell adhesion through regulation of cytoskeletion assembly.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Liver lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa40-80 of human ACP1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human Acid phosphatase 1, isoform b. Species Crossreactivity: bovine, porcine. Species sequence homology: mouse, rat, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bottini et.al 277 (27) 24220-24224 (July 5, 2002) 2. Giannoni et.al JBC 278 (38) 36763-36776 (September 19, 2003).