Technical Data
A0576-05
Acid Sensitive Ion Channel 3 (ASIC3, DRASIC, Dorsal Root Ganglion ASIC)
Description:
Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC), (3) and mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride. The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASIC for Acid Sensing Ion Channels). Three are at least three distinct proteins in ASIC family: ASIC1 (identical with human BNAC2 or BNC2), expressed in brain and dorsal root ganglions (DRG) cells, is activated by pH <7.0.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 using ECL.
ELISA: 1:10,000-1:50,000. Coat ELISA plates at 1ug/ml with Control Peptide.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
A0576-06: Acid Sensitive Ion Channel 3, Rat, CT, 21aa (ASIC3, DRASIC, Dorsal Root Ganglion ASIC) Control Peptide.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to 21aa sequence near the cytoplasmic C-terminus of rat DRASIC. (KLH coupled).
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes rat Acid Sensitive Ion Channel 3. No significant sequence homology with other ASICs. Species sequence homology: human: 95%; human testis specific DRASIC homolog hTNaCl: 90%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Waldmann, R., et al. (1997) J. Biol. Chem. 272: 20975-20978. 2. deWeille, J.R., et al. (1998) FEBS Lett. 433: 257-260. 3. Ishibashi, K., et al. (1998) BBRC 245: 589-593.