Technical Data
Acid Sensing Ion Channels 4 (ASIC4)
Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC); (3) mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N- and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride.

The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASICs for Acid Sensing Ion Channels). Three are at least 4 distinct proteins in ASIC family ASIC1-4. AISC4/SPASIC (human 539/666aa, chromosome 2; rat 539aa, ~97% homology between rat and human) is strongly expressed in pituitary and also detected in most areas of brain. ASIC4 is the most divergent member of ASIC family showing only 45% identity with ASIC1-3. ASIC4 is not activated by low pH. It may associate with other ASICs to form a functional channel.

Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL)
ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml with Control Peptide.
Optimal dilutions to be determined by the researcher.

Control Peptide:
A0577-10: Acid Sensing Ion Channels 4, Human (ASIC4) Control Peptide

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Synthetic peptide 19aa sequence of human ASIC4 (KLH coupled). Location: ~N-terminal; Extracellular. Species Sequence Homology: Rat, mouse: 100%.
Purified by immunoaffinity chromatography.
Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Recognizes human Acid Sensing Ion Channels 4 (ASIC4). No significant sequence homology is detected with other ASIC-beta or other ASICs.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Akopian, A.N., et al., Neuroprot. 14: 2217-2222 (2000). 2. Grunder, S., et al., Neuroprot. 14: 1607-1611 (2000).