Technical Data
A0578-83
Acidic Hair Keratin, K35 (KRT35, Keratin 35, HA5, Ha-5, Hair Keratin, Type I Ha5, hHa5, HHA5, HKA5, K35, Keratin, Type I Cuticular Ha5, Keratin-35, KRTHA5, hHa5)
Description:
The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2.

Applications:
Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.

Recommended Dilutions:
Western Blot (ECL): 1:2000
Immunohistochemistry (Frozen): 1:200. For enhancement of cortex staining, we recommend preincubating fixed sections with 0.1% Triton X-100 in PBS for 1-5 minutes prior to first antibody incubation step. Incubate for 1 hour at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanGuinea pig
Concentration:
Not determined
Immunogen:
Synthetic peptide of human acidic hair (trichocytic) keratin K35, YSSSPCKLPSLSPVARS (KLH).
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human hair keratin K35 expressed starting from the upper part of hair matrix, lower cortex and hair cuticle. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langbein, L., et al., J. Biol. Chem. 274: 19,874-19,893 (1999). 2. Langbein, L., et al., Methods in Cell Biology 78: 413-456 (2004).