Technical Data
A0578-87
Acidic Hair Keratin, K37 (KRT37, Keratin 37 HA7, Hair Keratin, Type I Ha7, hHa7, HHA7, HKA7, K37, Keratin, Type I Cuticular Ha7, Keratin-37, KRTHA7, hHa7)
Description:
Type I hair keratin is one of the two types of hair keratin. It is an acidic protein which heterodimerizes with type II hair keratins to form hair and nails.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:200. For enhancement of cortex staining, preincubate fixed sections with Triton X-100 (in PBS) for 1-5 minutes prior to first antibody incubation step. Incubate for 1 hour at RT.
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to human acidic hair (trichocytic) keratin K37, conjugated to KLH (Formally designated keratin hHa7; CGP VTG GSP SGH GAS MGR).
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide
Specificity:
Recognizes human hair keratin K37 expressed starting from the middle cortex of vellous hairs. Negative with cells in the anagen hair follicle. Species Crossreactivity: chimpanzee
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langbein, L., et al., J. Biol. Chem. 274: 19,874-19,893 (1999). 2. Jave-Suarez, L.F., et al., J. Invest. Dermatol. 122: 555-564 (2004). 3. Langbein, L., et al., Methods in Cell Biology 78: 413-456 (2004).