Technical Data
A0578-87
Acidic Hair Keratin, K37 (KRT37, Keratin 37 HA7, Hair keratin, type I Ha7, hHa7, HHA7, HKA7, K37, Keratin, type I cuticular Ha7, Keratin-37, KRTHA7, hHa7)
Description:
Type I hair keratin is one of the two types of hair keratin. It is an acidic protein which heterodimerizes with type II hair keratins to form hair and nails.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:200. For enhancement of cortex staining, preincubate fixed sections with Triton X-100 (in PBS) for 1-5 minutes prior to first antibody incubation step.
Western Blot (ECL): 1:2000 Incubate for 1 hour at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to human acidic hair (trichocytic) keratin K37, conjugated to KLH. (Formally designated keratin hHa7; CGP VTG GSP SGH GAS MGR)
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human hair keratin K37 expressed starting from the middle cortex of vellous hairs. Negative with cells in the anagen hair follicle. Species Crossreactivity: chimpanzee
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Langbein L, Rogers M, Winter H, Prätzel S, Beckhaus U, Rackwitz HR, Schweizer J: The catalog of human hair keratins: I. Expres- sion of nine type I members in the hair follicle. J Biol Chem 274, 19874-19893 (1999)2.Jave-Suarez LF, Langbein L, Winter H, Praetzel S, Rogers ML, Schweizer J : Androgen regulation of the human hair follicle : The type I hair keratin hHa7 is a direct target gene in trichocytes.3. J Invest Dermatol 122, 555-564 (2004)Langbein L, Spring H, Rogers MA, Praetzel S, Schweizer J: Hair keratins and hair follicle-specific epithelial keratins. Methods in Cell Biology 78, 413-456 (2004)