Technical Data
A0660-01D
Aconitase 1 (ACO1, ACONS, IREB1, IREBP, IREBP1, IRP1, OTTHUMP00000021176, OTTHUMP00000021177, OTTHUMP00000045233, aconitate hydratase, citrate hydro-lyase, ferritin repressor protein, iron regulatory protein 1, iron-responsive element binding protein 1)
Description:
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-QVDFNRRADSLQKNQ, from the internal region of human Aco1/Aconitase 1 (NP_002188.1)
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human Aco1/Aconitase 1. Sequence homology: mouse, rat, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang W, Di X, D'Agostino RB, Torti SV, Torti FM, Excess capacity of the iron regulatory protein system.
The Journal of biological chemistry 2007 Aug 282 (34): 24650-9.