Technical Data
ACO2, ID (Aconitate Hydratase, Mitochondrial, Citrate Hydro-lyase, Aconitase)
ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
Synthetic peptide selected from the central region of human Aconitase (KLH).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human ACO2. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Juang, H.H., Mol. Genet. Metab. 81(3):244-252 (2004). 2.Bota, D.A., et al., Nat. Cell Biol. 4(9):674-680 (2002). 3.Gruer, M.J., et al., Trends Biochem. Sci. 22(1):3-6 (1997). 4.Klausner, R.D., et al., Mol. Biol. Cell 4(1):1-5 (1993). 5.Geurts van Kessel, A.H., et al., Cytogenet. Cell Genet. 28(3):169-172 (1980).