Technical Data
Adiponectin, Human BioAssay™ ELISA Kit, High Sensitivity
Kits and Assays Storage: 4°C Do Not FreezeShipping: Blue Ice
This kit is a sandwich ELISA for the quantitative measurement of human adiponectin in serum, plasma, breast milk, urine, cerebrospinal fluid and tissue culture media. It is intended for in vitro and research use only.

• The total assay time is less than three hours.
• The kit measures total adiponectin.
• Standards are recombinant adiponectin based.
• Quality controls are human serum based. No animal sera are used.
• Components of the kit are ready-to-use (with the exception of Dilution Buffer and Wash Solution).

Kit Components:
A0725-52A: Microtiter Plate, 1x96 wells
A0725-52B: Adiponectin(HRP), 1x13ml
A0725-52C: High Control,1x50ul
A0725-52D: Low Control, 1x50ul
A0725-52E: Dilution Buffer (10X), 2x10ml
A0725-52F: Wash Solution (5X), 1x100ml
A0725-52G: Substrate (TMB) Solution, 1x13ml
A0725-52H: Stop Solution (0.2 M H2SO4), 1x13ml
A0725-52J: Standards 150ng/ml; 1x1ml
A0725-52K: Standards 100ng/ml 1x1ml
A0725-52L: Standards 50ng/ml 1x1ml
A0725-5M: Standards 20ng/ml 1x1ml
A0725-52N: Standards10ng/ml 1x1ml
A0725-52P: Standards 5ng/ml 1x1ml
A0725-52Q: Standards 2ng/ml 1x1ml
A0725-52R: Standards 1ng/ml 1x1ml

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application references: 1. Arismendi E., et al. 2014. PLOS One. 9(9):e107859. 2. Arismendi E., et al. Obes Surg. 2015 Jan 25. [Epub ahead of print].
Other References:1. Scherer, P.E., et al., J. Biol. Chem. 270: 26,746-26,749 (1995). 2. Maeda, K., et al., Biochem. Biophys. Res. Commun. 221: 286-289 (1996). 3. Arita, Y., et al., Biochem. Biophys. Res. Commun. 257: 79-83 (1999). 4. Das, K., et al., Biochem. Biophys. Res. Commun. 280: 1120-1129 (2001). 5. Wang, Y., et al., J. Biol. Chem. 277: 19,521-19,529 (2002). 6. Kondo, H., et al., Diabetes 51: 2325-2328 (2002). 7. Spranger, J., et al., Lancet 361: 226-228 (2003). 8. Pajvani, U.B., et al., J. Biol. Chem. 278: 9073-9085 (2003). 9. Yamauchi, T., et al., J. Biol. Chem. 278: 2461-2468 (2003). 10. Yamauchi, T., et al., Nature 423: 762-769 (2003). 11. Waki, H., et al., J. Biol. Chem. 278: 40,352-40,363 (2003). 12. Ishikawa, Y., et al., Histopathology 42: 43-52 (2003). 13. Ouchi, N., et al., J. Biol. Chem. 279: 1304-1309 (2004).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.