Technical Data
Actin, beta (Non-muscle), Control Peptide
Molecular Biology Storage: -20CShipping: Blue Ice
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g- smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.

Synthetic peptide corresponding to N-terminal 15aa of human beta-actin, non-muscle.

Suitable for use in Antibody Blocking and ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source: Synthetic peptide
Purity: Purified
Concentration: As reported
Form: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ohmuri H (1995) Gene Accession # S38782; 2. Vandekerchove, J et al (1978) Eur. J. Biochem. 90, 451; 3. Lessard J et al (1988) Cell. Motil Cytoskel. 10, 349; 4. North JA et al (1994) J. Cell Sci. 107, 437.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.