Technical Data
A0855-39G
Activation Transcription Factor 1 phosphorylated (Ser63) (ATF1, TREB-36)
Description:
ATF-1 is a cAMP-responsive enhancer (CRE) binding protein that is expressed in a wide variety of cell types and is capable of dimerizing with CREB. ATF-1 contains a consensus phosphorylation site for PKA and shares extensive homology with CREB in the region surrounding and carboxyl-terminal to the PKA site (1). Epidermal growth factor induction of c-jun expression requires ATF-1 and MEF2 sites in the c-jun promoter. Activation of the c-jun promoter through the ATF-1 site requires phosphorylation of ATF-1 at serine 63. Two MAPK pathways, ERK and JNK, are required for EGF-induced c-jun expression and the ERK pathway acts through downstream phosphorylation of ATF-1 (2).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50,000-1:100,000
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:100-250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E229Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF-1.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Specificity:
Recognizes ATF-1 phosphorylated on serine 63.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rehfuss RP, et al J Biol Chem 266(28):18431-4, 1991. 2. Gupta P et al. J Biol Chem (52):50550-6, 2002