		Technical Data
A0855-94B	Activin C

Description:
Activins belong to the TGF-beta superfamily. They are homo- or heterodimers of activin beta subunits. At least four mammalian beta subunits, A, B, C, and E, have been described. The beta c subunit homodimerizes to form Activin CC. A naturally occuring Activin AC heterodimer has also been reported.

Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~10ng/well.
Western Blot: 1-2ug/ml. The detection limit is ~25ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma with B cells obtained from a mouse

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	9H18	Affinity Purified

Size		Storage	Shipping	Source	Host
500ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml (after reconstitution)

Immunogen:

Recombinant corresponding to human Activin C expressed in E. coli.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.

Specificity:

Recognizes human Activin C.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mellor, S. et al., 2003, Endocrinology 144:4410.