Technical Data
A0855-94B
Activin C
Description:
Activins belong to the TGF-beta superfamily. They are homo- or heterodimers of activin beta subunits. At least four mammalian beta subunits, A, B, C, and E, have been described. The beta c subunit homodimerizes to form Activin CC. A naturally occuring Activin AC heterodimer has also been reported.

Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~10ng/well.
Western Blot: 1-2ug/ml. The detection limit is ~25ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma with B cells obtained from a mouse

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19H18Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant corresponding to human Activin C expressed in E. coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity:
Recognizes human Activin C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mellor, S. et al., 2003, Endocrinology 144:4410.