Technical Data
ACVR2B, NT (Activin Receptor Type 2B, Activin Receptor Type IIB, ACTR-IIB)
Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. ACVR2B (activin A type IIB receptor) displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ul-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to a portion of amino acids within aa 1-30 from the N-terminal region of human ACVR2B, conjugated to KLH (Q13705)
Purified by ammonium sulfate precipitation.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human ACVR2B. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Harrison, C.A., et al., J. Biol. Chem. 279(27):28036-28044 (2004). 2.Martins da Silva, S.J., et al., Dev. Biol. 266(2):334-345 (2004). 3.Casagrandi, D., et al., Mol. Hum. Reprod. 9(4):199-203 (2003). 4.Shin, B.K., et al., J. Biol. Chem. 278(9):7607-7616 (2003). 5.Schneider-Kolsky, M.E., et al., Placenta 23(4):294-302 (2002).