Technical Data
A0857-43
ACTR1B (ARP1, ARP1B, Centractin Beta (Yeast), CTRN2, PC3, Yeast Homolog B)
Description:
In addition to conventional actins, there are several actin-related proteins. The ACTR1B protein and the ACTR1A protein are of equal length, and they share 90% amino acid identity and 96% amino acid similarity. ACTR1B is expressed in the cytosol as part of the dynactin complex. The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1B appears to be ivolved in dynein-driven vesicle movement.

Cellular Localization: Cytoplasmic

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:128000.
Western Blot: 0.5-2ug/ml. Predicted molecular weight 45kD. Detects a band of ~ 43kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide: YEEDGSRAIHRKT representing the C terminus of the human protein (residues 363-375),according to NP_005726.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
This antibody may crossreact with ACTR1A. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse and Rat due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Clark SW et al. Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins. Mol Biol Cell 5:1301-10 (1994).