Technical Data
A0859-26B
ADAM 12S, Recombinant, Human, Western Blot Positive Control (Disintegrin and Metalloproteinase Domain-containing Protein 12, ADAM 12, Short Form, Meltrin-alpha,UNQ346/PRO545 MLTN)
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
ADAM proteins are a family of membrane-anchored cell surface protein containing both a disintegrin and a metalloprotease domain. Their exact functions have not been fully characterised however, they are thought to mediate a number of cellular processes including differentiation, cell fusion, cell-cell interactions and shedding of plasma membrane bound growth factor precursors. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form (ADAM12S) is a soluble form, which lacks the transmembrane and cytoplasmic domains. ADAM12S expression is thought to be limited to the placenta, embryo and foetus although levels have been detected in some tumor cell lines.

Source:
Recombinant corresponding to aa208-738 from human ADAM12S, fused to N-terminal 6x His-tag, expressed in E. coli.

Applications:
Suitable for use in Western Blot as a control. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: Purified (SDS-PAGE)
Concentration: As reported
Form: Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Primakoff, P. & Myles D.G. (2000) Trends Genetics 16 (2): 83-87.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.