Technical Data
A0859-35H
ADAM 17, CT (ADAM17, A Disintegrin and Metalloproteinase Domain 17 (Tumor Necrosis Factor, alpha, Converting Enzyme), CSVP, TACE, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM Metallopeptidase Domain 17, TNF-alpha Converting Enzyme, A Disintegrin and Met
Description:
Alternate Name: ADAM17, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), CSVP, TACE, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM metallopeptidase domain 17, TNF-alpha converting enzyme, a disintegrin and metalloproteinase domain 17, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), snake venom-like protease

Domain Information: Disintegrin; Homologues of snake disintegrins; Reprolysin (M12B) family zinc metalloprotease

Gene Ontology Terms: metallopeptidase; cell-cell signalling; integral plasma membrane protein; peripheral plasma membrane protein

Application(s):
Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 125-130kD band observed in Hela, Jurkat and U937 lysates (predicted MW of 95kD according to NP_003174.3 how ever 130kD forms have been reported elsewhere, probably due to glycosylation).

Staining (0.2ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See A0859-35H-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
LQRQNRVDSKETEC, C Terminus
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
This antibody is expected to recognize isoform 1 (represented by NP_003174) and not isoform 2 (represented by NP_068604). Species Crossreactivity: human, mouse, rat, Chinese hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Black RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, Castner BJ, Stocking KL, Reddy P, Srinivasan S, Nelson N, Boiani N, Schooley KA, Gerhart M, Davis R, Fitzner JN, Johnson RS, Paxton RJ, March CJ, Cerretti DP, A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells, Nature. 1997 Feb 20;385(6618):729-33