Technical Data
A0859-42B
ADAM 33 (A Disintegrin and Metalloprotease 33, A Disintegrin and Metalloproteinase Domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889, Disintegrin and Reprolysin Metalloproteinase Family Protein, Metalloprotease Disintegrin)
Description:
ADAM33 is a widely expressed member of the ADAM family of transmembrane proteins. It contains a metalloprotease-like domain, disintegrin cell adhesion domain, and cysteine rich domain. ADAM33 is involved in the maintenance of airway function, and ADAM33 polymorphisms have been associated with asthma susceptibility and bronchial hyperresponsiveness. Within the mature ectodomain, human and mouse ADAM33 share 79% amino acid sequence identity.

Applications:
Suitable for use in Flow Cytometry, Direct ELISA, and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 25ug/ml. 10ul labels 1-5x10e5 A549 cells in a total reaction volume 200ul. For intracellular staining, cells must first be fixed and permeabilized using 4% paraformaldehyde, 0.1% saponin in PBS.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human ADAM33 is ~10ng/well.
Immunoprecipitation: 25ug/ml to immunoprecipitate recombinant human ADAM33 from conditioned cell culture media.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19H38Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa30-501 from human ADAM33, expressed in CHO cells (Q9BZ11).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes human ADAM33. Species Crossreactivity: recombinant mouse ADAM33, <5%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.