Description:
Applications:
Suitable for use in Immunofluorescence microscopy, Immunohistochemistry, Immunoprecipitation, Immunoblot and ELISA.
Recommended Dilution:
Immunohistochemsitry: 1:10-1:20; Incubation Time 3h at room temperature or overnight at 2-8°C.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a | 5F146 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 50ug | | -20°C | Blue Ice | | Mouse |
|
| Concentration: |
| ~0.05mg/ml |
| Immunogen: |
| Adeno-associated virus capsid proteins and virus particles. |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a lyophilized powder with 0.5% BSA and 0.09% sodium azide. Reconstitute to 1ml final volume. |
| Specificity: |
| Antigen recognized in Species AAV-2, found in human and monkey (tested so far). Weak crossreaction with serotypes 1, 3, 4, 5 and 6. Reacts with VP1 of adeno-associated virus. In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found. Epitope mapping experiments identified aa123 to aa131 of VP1 capsid protein as the specific binding region. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Wistuba A., Kern A., Weger S., Grimm D., and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352. Wobus C.A., Hügle-Dörr B., Girod A., Petersen G., Hallek M. and Kleinschmidt J.A. (2000) Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virology 74, 9281-9293
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