Technical Data
A0868-36C
Adeno-Associated Virus 2, VP1 (AAV2)
Description:
Applications:
Suitable for use in Immunofluorescence microscopy, Immunohistochemistry, Immunoprecipitation, Immunoblot and ELISA.

Recommended Dilution:
Immunohistochemsitry: 1:10-1:20; Incubation Time 3h at room temperature or overnight at 2-8C.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.


TypeIsotypeCloneGrade
MabIgG2a5F146Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouse
Concentration:
~0.05mg/ml
Immunogen:
Adeno-associated virus capsid proteins and virus particles.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder with 0.5% BSA and 0.09% sodium azide. Reconstitute to 1ml final volume.
Specificity:
Antigen recognized in Species AAV-2, found in human and monkey (tested so far). Weak crossreaction with serotypes 1, 3, 4, 5 and 6. Reacts with VP1 of adeno-associated virus. In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found. Epitope mapping experiments identified aa123 to aa131 of VP1 capsid protein as the specific binding region.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wistuba A., Kern A., Weger S., Grimm D., and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352. Wobus C.A., Hgle-Drr B., Girod A., Petersen G., Hallek M. and Kleinschmidt J.A. (2000) Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virology 74, 9281-9293