Technical Data
A0869-59
Adenosine Receptor A2A
Description:
Adenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000. Detects Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.
Immunohistochemistry: 1ug/ml. Detects Adenosine Receptor A2A in paraformaldehyde-fixed mouse brain striatal sections. Not detected in Adenosine Receptor A2A knockout mice.
Flow Cytometry: Detects Adenosine Receptor A2A in transfected HEK293 cells.
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse brain striatal sections

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a5H30Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
6His-tagged full-length human recombinant A2A receptor. Epitope: third intracellular loop (SQPLPGER). (NM_000675)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.01M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human adenosine receptor A2A at ~43kD. Species Crossreactivity: mouse, rat, rabbit and canine. Crossreactivity with guinea pig also reported.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rosin, D.L., et al., J. Comp. Neurol. 401: 163-186 (1998). 2. Hettinger, B.D., et al., J. Comp. Neurol. 431: 331-346 (2001). 3. Koshiba, M., et al., Mol. Pharmacol. 55: 614-624 (1999).