Technical Data
Adenosine Receptor A2A
Adenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases.

Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000. Detects Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.
Immunohistochemistry: 0.2ug-1ug/ml. Detects Adenosine Receptor A2A in paraformaldehyde-fixed mouse brain striatal sections. Not detected in Adenosine Receptor A2A knockout mice.
Flow Cytometry: Detects Adenosine Receptor A2A in transfected HEK293 cells.
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse brain striatal sections

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a5H30Affinity Purified
200ug-20CBlue IceHumanMouse
6His-tagged full-length human recombinant A2A receptor (NM_000675). Epitope: third intracellular loop (SQPLPGER).
Purified by Protein G affinity chromatography.
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Recognizes human adenosine receptor A2A at ~43kD. Species Crossreactivity: mouse, rat, rabbit and canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rosin, D.L., et al., J. Comp. Neurol. 401: 163-186 (1998). 2. Hettinger, B.D., et al., J. Comp. Neurol. 431: 331-346 (2001). 3. Koshiba, M., et al., Mol. Pharmacol. 55: 614-624 (1999).