Technical Data
A0869-66
Adenosine A2b Receptor
Description:
Adenosine A2b Receptor is a G-protein coupled adenosine receptor. It is an integral, multi-pass membrane protein which activates adenylate cyclase activity in the presence of adenosine.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Not suitable for use in Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Frozen, formalin fixed): 10-50ug/ml.
Western Blot: 1-10ug/ml (ECL), 40-100ug membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
ELISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.

Positive Controls:
Epithelial cells of the crypts, Synctiotrophoplast cells of human placenta, T84 human colon adenocarcinoma cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corrsponding to a 16aa sequence of the 2nd extracellular domain of the human brain A2bR gene (1-3).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes human Adenosine A2b receptor. Recognizes a band of ~50-52kD in several rat, mouse and human tissues (thymus, placenta, colon and small intestine). Also recognizes the 35kD band in small intestine. Does not recognize the native receptors. No bands were detected in skeletal muscle or heart. Will not recognize native protein in immunoprecipitation experiments. Species crossreactivity: rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. BBRC (1992) 187:86-93. 2. Cardiovascular Res. (1993) 27:62-67. 3. Ann. Rev. Pharmacol. (1995) 35:581-606. 4. Mol. Pharmacol. (1995) 47:1126-1132.