Technical Data
A0876-48
Adenovirus
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Technical Data |
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A0876-48 |
Adenovirus |
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Description: The adenovirus early gene products E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth inhibitory effects of TGF beta. The E1A region encodes a series of related proteins (35-46kD) with multifunctional capabilities and forms a specific complex with the retinoblastoma tumor suppressor gene product. The E1a and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Application(s): Immunohistochemistry (formalin fixed paraffin embedded / frozen sections). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Dilution: Immunohistochemistry (formalin fixed paraffin embedded / frozen sections): 1:50-1:75 in an ABC method Positive Control: Suitable tissue control sections for Immunohistochemistry:Adenovirus infected tissue. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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