Technical Data
A0880-19AX
Adenovirus Fiber (Monomer and Trimer) (BSA & Azide Free)
Description:
Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the typespecific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron.

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control:
Adenovirus infected cells and tissues.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k3F13Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceMouse
Concentration:
~1mg/ml
Immunogen:
UV-irradiated Ad2 virus. MW of Antigen: 62kD (monomer) and 180-200kD (trimer). Epitope: N-terminus, probably the first 17aa. Cellular Localization: Nuclear.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See A0880-19A
Specificity:
Recognizes both monomers and trimers, as judged by reactivity with both boiled (monomers (62kD) only) and unboiled (both monomers and trimers (180200kD bands)) fiber on Western blots and by indirect immunofluorescence studies of cells infected with a temperature-sensitive Ad5 fiber mutant (H5ts142) which does not form trimers at the nonpermissive temperature at both 320C and 39.50C.(1) Species Crossreactivity: Adenovirus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Key References: 1. Hong, J.S., Engler, J.A. Virology., 185, 758767, 1991.Material Safety Data: 2. Hong, J.S., Engler, J.A. J. Virology, 70, 70717078, 1996.