Technical Data
A0890
Adipsin
Description:
Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology. Adipsin is a serine protease that is secreted by adipocytes. It is deficient in several animal model of obesity. Adipsin has now been identified as the same protein as complement factor D.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot:1-5 ug/ml using ECL. Adipsin is ~22 kD
ELISA: 1:10,000-50,000. Coat 10-100ng of A0890-01 control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide:
A0890-02: Adipsin, Human (Control Protein for Western Blot)
A0890-01: Adipsin (Blocking Peptide, ELISA Standard)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to (18aa) internal peptide sequence of human adipsin conjugated to KLH (P00746). Species Sequence Homology: chimp: 95%, bovine: 80%, porcine: 78%; rat: 70%; mouse: 63%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes human Adipsin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhu, L., et al., J. Clin. Nvest. 94: 1163 (1994). 2. Baker, B.C., et al., 279: 775 (1991). 3. Min, H.Y., et al., Nucl Acid Res. 14: 8879. 5. White, R.T., et al., JBC 267: 9210 (1992). 6. Niemann, M.A., et al., Biochem. 23: 2482 (1984). 7. Johnson, D.M., et al., Bicohem. J. 187: 863 (1980). 8. Volanakis, J.E., et al., PNAS 77: 1116 (1980).