Technical Data
A0890-03
Adipsin
Description:
Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology. Adipsin is a serine protease that is secreted by adipocytes. It is deficient in several animal model of obesity. Adipsin has now been identified as the same protein as complement factor D. Adipsin, also called ADN or complement factor D or C3 convertase activator or properdin factor D (precursors: mouse 259aa; rat 263aa, human 253aa, mature protein 26-253, ~22kD) cleaves factor B when the latter is complexed with factor C3B, activating the C3BB complex, which then becomes C3 convertase of the alternative pathway. Adipsin is the major protein secreted by the adipocytes. Unlike rodents, adipsin is also expressed in monocytes/macrphages. Most adipsin is secreted in blood (50ug/ml in normal lean mice and 50-100 fold less in fat from db/db or ob/ob or MSG (monosodium glutamate-treated mice). Its expression is induced upon differentiation of preadipocytes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-5 ug/ml using ECL.
ELISA: 1:10,000-50,000. Coat 10-100ng of A0890-04 control peptide/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 14aa sequence near the N-terminus of rat adipsin conjugated to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity:
Recognizes rat adipsin at ~22kD. Species Sequence Homology: mouse: 65%. Recommend using human adipsin (A0890 or A0890B) for human studies.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhu, L., et al., J. Clin. Nvest. 94: 1163 (1994). 2. Baker, B.C., et al., 279: 775 (1991). 3. Min, H.Y., et al., Nucl Acid Res. 14: 8879. 5. White, R.T., et al., JBC 267: 9210 (1992). 6. Niemann, M.A., et al., Biochem. 23: 2482 (1984). 7. Johnson, D.M., et al., Bicohem. J. 187: 863 (1980). 8. Volanakis, J.E., et al., PNAS 77: 1116 (1980).