		Technical Data
A0901-25	ADPN (Adiponutrin, Patatin-like Phospholipase Domain containing 3)

Description:
Adiponutrin is one of three recently identified adipocyte lipases. Surprisingly, these proteins also retain transacylase activity, a hitherto unknown pathway of triacylglycerol synthesis in the adipocytes. This may enable them to participate in both anabolic and catabolic processes. The adiponutrin gene (ADPN) is downregulated by fasting and upregulated by refeeding, suggesting a role in lipogenesis. Experiments in human adipocytes confirmed that the gene is upregulated in response to insulin in a glucose-dependent fashion. Obese subjects had increased levels of subcutaneous and visceral abdominal adipose tissue ADPN mRNA. Visceral ADPN mRNA expression was correlated to measures of insulin sensitivity (fasting insulin and homeostasis model assessment).

Genbank accession Number: BC014449
Alternative Symbols: PNPLA3,iPLA(2)epsilon
Calculated Molecular Weight: 481aa,53kD
Observed Molecular Weight: 53kD

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1: 1000-1: 100,000
Western Blot: 1: 100-1: 1000.
Optimal dilution determined by the researcher

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
150ul		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

~0.1mg/ml

Immunogen:

Recombinant human ADPN.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide., 50% glycerol.

Specificity:

Recognizes human ADPN (Adiponutrin,Patatin-like Phospholipase Domain containing 3) at 53kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Diabetes. 2006 Mar;55(3):826-33, Variation in the adiponutrin gene influences its expression and associates with obesity. Johansson LE, Hoffstedt J, Parikh H, Carlsson E, Wabitsch M, Bondeson AG, Hedenbro J, Tornqvist H, Groop L, Ridderstråle M.