Technical Data
A0901-25
ADPN (Adiponutrin, Patatin-like Phospholipase Domain containing 3)
Description:
PPNPLA3(patatin-like phospholipase domain-containing protein 3), also named as ADPN, C22orf20, alternatively referred to as adiponutrin, encodes a 481-amino acid protein of 54 kD that belongs to the patatin-like phospholipase domain-containing family. Despite the apparent absence of a membrane-spanning domain, PNPLA3 is tightly associated with membranes and it may be targeted specifically to regions of the ER membrane that are destined to become nascent lipid droplets (PMID:22338072). It is a multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. Genetic variations in PNPLA3 are a cause of susceptibility to non-alcoholic fatty liver disease type 1 (NAFLD1). It has 2 isoforms produced by alternative splicing

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:200-1:1000
Optimal dilution determined by the researcher

Positive Controls:
HepG2 cells, human adipose tissue and Jurkat cells

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
150ul-20°CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
PNPLA3 fusion protein ag1959
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.3, 0.1% sodium azide, 50% glycerol.
Specificity:
Recognizes human ADPN at ~53kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Diabetes. 2006 Mar;55(3):826-33, Variation in the adiponutrin gene influences its expression and associates with obesity. Johansson LE, Hoffstedt J, Parikh H, Carlsson E, Wabitsch M, Bondeson AG, Hedenbro J, Tornqvist H, Groop L, Ridderstråle M.