Technical Data
ADP Ribosylation Factor (ARF)
The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21kD proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Suitable for use in Immunofluorescence, Immunoprecipitation, Immunocytochemistry and Western Blot. Cannot be used to neutralize Arf in solution. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:500. Staining of Arf in rat pancreas results in staining of the cytoplasmic face of the trans-Golgi membranes.
Western Blot: 1:500. Detects a single ~21kD protein representing Arf in rat pancreas extract
Immunoprecipitation: 1:50
Optimal dilutions to be determiend by the researcher.

Cellular Localization: Golgi apparatus

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

100ul4C (-20C Glycerol)Blue IceHumanMouse
Not Determined
Recombinant human Arf 1
Supplied as a liquid in PBS, 0.05% sodium azide.
Recognizes human ADP-ribosylation factor1 (Arf 1), Arf 3, Arf 5 and Arf 6 and at about a ten-fold reduction with Arf 4. Species Crossreactivity: rat, mouse, canine and hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
J. Biol. Chem., 271(36): 21767-21774, 1996. General: Cell, 67: 239-253, 1991 .J. Cell Bio.,140(3): 511-523, 1998. J. Cell Biol., 138(3): 505-515, 1997. Proc. Natl. Acad. Sci. USA, 89; 6619-6623, 1992. Trends in Biochemical Sciences, 20: 147-150, 1995.