Technical Data
AES, CT (Amino Enhancer of Split, Amino-terminal Enhancer of Split, AES 1, AES-1, AES 2, AES-2, Esp1, Gp130-associated Protein GAM, Groucho Homolog, GRG, GRG Protein, GRG5, Protein ESP1, TLE5)
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control: human 293 cell lysates

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug4C/-20C (No Glycerol)Blue IceHumanRabbit
A 16aa peptide located near human AES carboxyterminus.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human AES (C-TERMINAL). Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Miyasaka, H. et al. (1993) Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 216: 343352. 2. Jan, Y. et al. (2004) A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors. Cell. 116: 751762. 3. Tetsuka, T. et al. (2000) Inhibition of nuclear factorkappaBmediated transcription by association with the aminoterminal enhancer of split, a Grouchorelated protein lacking WD40 repeats*. J. Biol. Chem. 275: 43834390.