Technical Data
Aflatoxin B1, Food, BioAssay™ ELISA Kit
Kits and Assays Storage: 4°CShipping: Blue Ice
Aflatoxins belong to the class of mycotoxins. Chemically they are defined as difuranocyclopentanocumarines or difuranopentanolidocumarines, i.e. aflatoxins contain a dihydrofuran or a tetrahydrofuran ring, to which a substituted cumarin system is condensed. Out of about 20 known aflatoxins, the molds Aspergillus flavus and A. parasiticus produce exclusively aflatoxin B1, B2, G1 and G2, and all the other aflatoxins are derivates of these four. The derivates are developed either by metabolism in humans, animals and microorganisms or by environmental reactions.
Aflatoxins belong to the strongest mycotoxins, which act primarily in a hepatotoxic and carcinogenic way. The four main aflatoxins show a different toxicity. B1 is without doubt the most toxic aflatoxin, followed by G1, B2 and G2. Aflatoxin B1, however, does not show a direct toxic action. In the process of biotransformation in the liver, the lipophilic toxin is epoxidated and transformed into an active derivative, the so-called aflatoxin B1-2, 3-epoxid. This highly reactive epoxid is able to react with nucleophilic regions of macromolecules. Amongst other this metabolite of aflatoxin B1 binds covalently to the N-7 atom of the guanine bases of DNA. This covalent bond causes an inhibition of the DNA replication, the RNA synthesis and mutations.
Both chronical and acute intoxications are effected by aflatoxins. There are only few documented reports about acute intoxications, which are caused by uptake of mycotoxins. Of special importance for human beings are the chronical intoxications by aflatoxins. To the diseases, which develop after such chronical intoxications, belong primary liver carcinoma, hepatitis, Reye’s syndrome and Kwashiorkor. Besides the generation of primary liver carcinoma, aflatoxins are presumably also responsible for other sorts of tumors, like intestinal cancer.
Contaminations with aflatoxins occur mostly with nuts and grain. In most cases aflatoxins penetrate the human body via the food. Aflatoxins are stable to heat and are only partly destroyed by boiling. In order to protect people against aflatoxin-induced diseases, there is a need for the quantitative and qualitative control of endangered foodstuff, besides appropriate hygienic precautions, which avoid the formation of aflatoxins. The United States Biological Aflatoxin B1 ELISA is a quick, economical and sensitive method to detect aflatoxin B1 in food. After an appropriate sample preparation, 40 samples can be tested in duplicate within 140 minutes.

Intended Use: For the quantitative determination of Aflatoxin B1 in food.

Sensitivity: 5pg/ml

Recovery (Spiked Samples): ~80%

Incubation Time: 140 min

Kit Components:
A0925-08A: Microtiter plate 1x96 wells each, coated with aflatoxin conjugate
A0925-08B: Standard 10X (0pg/ml), 1x500ul each in methanol
A0925-08C: Standard 10X (100pg/ml), 1x500ul each in methanol
A0925-08D: Standard 10X (400pg/ml), 1x500ul each in methanol
A0925-08E: Standard 10X (1000pg/ml), 1x500ul each in methanol
A0925-08F: Standard 10X (4000pg/ml), 1x500ul each in methanol
A0925-08G: Standard 10X (10,000pg/ml), 1x500ul each in methanol
A0925-08H: Anti-Aflatoxin B1 Antibody (rabbit), 1x6ml, ready to use
A0925-08J: Pab gG (HRP), 1x15ml, ready to use
A0925-08K: Substrate Solution (TMB) 1x15ml, ready to use
A0925-08L: Stop Solution (0.5M H2SO4), 1x15ml, ready to use
A0925-08M: Sample/Standard Diluent (PBS), 1x60ml, ready to use
A0925-08N: Wash Solution, 10X (PBS + Tween 20), 1x60ml; Dilute 1:9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up to 37°C for 15 minutes.

Storage and Stability:
Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. van Egmond, H.P. & Wagstaffe, P.J., Food Addit. Contam. 7: 239-251 (1990). 2. Beaver, R.W., et al., Lin, J. Assoc. Off. Anal. Chem. 74: 827-829 (1991) 3. Horwitz, W., et al., J. AOAC Int. 76: 461-491 (1993). 4. Biogene Gifte - Biologie, Chemie, Pharmakologie, 2. Auflage (Eds.: E. Teuscher, U. Lindequist), Gustav Fischer Verlag, Stuttgart, 1994. 5. Beti, J.A.., et al., J. Econ. Entomol. 88: 1776-1782 (1995). 6. Vasanthi, S. & Bhat, R.V., Indian J. Med. Res. 108: 212-224 (1998). 7. Gathumbi, J.K., et al., Lett. Appl. Microbiol. 32: 349-351 (2001). 8. Otteneder, H. & Majerus, P., Dtsch. Lebensm. Rdsch. 97: 334-338 (2001).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.