Technical Data
A0925-09
Aflatoxin M1, Milk, BioAssay™ ELISA Kit
1Kit
Kits and Assays Storage: 4°CShipping: Blue Ice
The United States Biological Aflatoxin M1 ELISA is a quick, economical, and sensitive method to detect aflatoxin M1 in milk and milk products. After an appropriate sample preparation, 40 samples can be tested in duplicate within 140 minutes.

Sample Type:
Milk and milk products.

Intended Use:
For the quantitative determination of Aflatoxin M1 in milk and milk products.

Sensitivity:
<10pg/mL

Recovery (Milk): ~102%

Recovery (Cheese): ~60%

Incubation Time: 140 min

Kit Components:
A0925-09A: Microtiter plate, 1x96 wells
A0925-09B: Aflatoxin M1 Standard (0pg/ml), 1x500ul
A0925-09C: Aflatoxin M1 Standard (100pg/ml), 1x500ul
A0925-09D: Aflatoxin M1 Standard (500pg/ml), 1x500ul
A0925-09E: Aflatoxin M1 Standard (1000pg/ml), 1x500ul
A0925-09F: Aflatoxin M1 Standard (5000pg/ml), 1x500ul
A0925-09G: Aflatoxin M1 Standard (10,000pg/ml), 1x500ul
A0925-09H: Aflatoxin M1 Antibody, 1x 6ml
A0925-09J: IgG (HRP), 1x15ml
A0925-09K: Substrate (TMB), 1x15ml
A0925-09L: Stop Solution (0.5M H2SO4), 1x15ml
A0925-09M: Sample Buffer (PBS), 1x60ml
A0925-09N: Wash Buffer (10X), 1x60ml

Storage and Stability:
Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. C. Schlatter, J Environ Pathol Toxicol Oncol., (1990) 10, 138-144: Past and future in mycotoxin toxicology research. 2. H. S. el-Nezami, G. Nicoletti, G. E. Neal, D.C. Donohue, J. T.Ahokas, Food Chem Toxicol., (1995) 33, 173-179: Aflatoxin M1 in human breast milk samples from Victoria, Australia and Thailand. 3. I. Aman, Zentralbl Veterinärmed. [B] (1992) 39, 692-694: Reduction of aflatoxin M1 in milk using hydrogen peroxide and hydrogen peroxide plus heat treatment. 4. W. Horwitz, R. Albert, S. Nesheim, J AOAC Int. (1993) 76, 461-491: Reliability of mycotoxin assays-an update. 5. H. P. van Egmond, Food Addit Contam. (1995) 12, 321-330: Mycotoxins: regulations, quality assurance and reference materials. 6. P. Markaki, E.Melissari, Food Addit Contam. (1997) 14, 451-456: Occurrence of aflatoxin M1 in commercial pasteurized milk determined with ELISA and HPLC. 7. P. M. Scott, M. W. Trucksess, J AOAC Int. (1997) 80, 941-949: Application of immunoaffinity columns to mycotoxin analysis. 8. L. Sibanda, S. De Saeger, C. Van Peteghem, Int J Food Microbiol. (1999) 48, 203-209:Development of a portable field immunoassay for the detection of aflatoxin M1 in milk. 9. E.K. Kim, D.H. Shon, D. Ryu, J.W. Park, H.J. Hwang, Y.B. Kim, Food Addit Contam. (2000) 17, 59-64: Occurrence of aflatoxin M1 in Korean dairy products determined by ELISA and HPLC. 10. F. Galvano, V. Galofaro, A. Ritieni, M. Bognanno, A. De Angelis, G. Galvano, Food Addit Contam., (2001) 18, 644-646: Survey of the occurrence of aflatoxin M1 in dairy products marketed in Italy: second year of observation. 11. H. Otteneder, P. Majerus, Dtsch. Lebensm. Rdsch. (2001) 97, 334-338: Mykotoxine, Lebensmittel-Kontaminanten Nr. 1? - Versuch einer Standortbestimmung.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.