Technical Data
Media
Agar, USP
A0930-05
Molecular Biology Grade
Storage RT    Shipping RT

Pharmaceutical Grade (USP) agar is a highly purified extract of select Gelidium marine algae, forming part of the cell wall, that gives excellent growth characteristics used in a wide variety of microbiological applications.

Agar is a phycocolloid, a complex mixture of several sugar compounds that retains a gelatinous structure at room temperature after boiling. Bacteriological agar is purified by successive washings to remove or extensively reduce chemical contaminants, color and salts.

After autoclaving, agar is a light amber and free of particulates, solidifying into a clear, firm gel and suitable for all molecular biology applications. Bacterial and phage growth (M13 and Lambda) is superior to standard microbiology grade agars.


Appearance: White to light tan, homogenous, free flowing powder.

Melting Point (1.5%): 82-88C

pH (1.5%): 6.0-8.0

Water: 11%

Ash: 6.5%

Gel Point (1.5%): 34-38C

Gel Strength (1.5%): 600-1100g/cm2

Identification A: Passes

Identification B: Passes

Foreign Insoluble Matter: 10%

Foreign Organic Matter: 1%

Foreign Starch: None detected

Gelatin: None detected

Water Absorption: 75ml

Acid-insoluble Ash: 0.5%

Arsenic: 0.0003%

Lead: 0.001%

Heavy Metals (Pb): 0.004%

Microbiological Analysis: Total Plate Count


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Falk, Bucca and Simmons, J. Bacteriology, 37: 121 (1939) 2. Hitchens and Leikind, J. Bacteriology, 37: 485 (1939) 3. Seip, In Fogg and Jones (ed), Proc. 8th Intl. Seaweed Symp. (1981) 4. United States Pharmacopeia, 8/14/12: Changed
Lead: 0.001%
-LP

04/21/11- Added Identification a,b, matter, starch, gelatin, absorption, ash.
-JE

2/9/10: Changed
Description:
Gelidium based Drosophila agar is an inexpensive growth medium especially suited to the growth and nutrient requirements of Drosophila without the expense of bacteriological grades. This is a food grade agar which offers excellent clarity.
-LP
5/15/09-remove: To Pass USP24/NF19
-add Exceed USP Specifications
-DF

04/07/05-Updated to read similar: A0930, -05.
-Changed Appearance: White to cream colored powder
-Changed pH (1.5%): 6.0-7.5
-VV23: 1721,1897 (1990)