Technical Data
Media
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Technical Data
Media
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Agar, USP
A0930-05
Molecular Biology Grade
Storage RT Shipping RT
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Pharmaceutical Grade (USP) agar is a highly purified extract of select Gelidium marine algae, forming part of the cell wall, that gives excellent growth characteristics used in a wide variety of microbiological applications. Agar is a phycocolloid, a complex mixture of several sugar compounds that retains a gelatinous structure at room temperature after boiling. Bacteriological agar is purified by successive washings to remove or extensively reduce chemical contaminants, color and salts. After autoclaving, agar is a light amber and free of particulates, solidifying into a clear, firm gel and suitable for all molecular biology applications. Bacterial and phage growth (M13 and Lambda) is superior to standard microbiology grade agars. Appearance: White to light tan, homogenous, free flowing powder. Melting Point (1.5%): 82-88°C pH (1.5%): 6.0-8.0 Water: 10% Ash: 6.5% Gel Point (1.5%): 34-38°C Gel Strength (1.5%): 600-1100g/cm2 Identification A: Passes Identification B: Passes Foreign Insoluble Matter: 10% Foreign Organic Matter: 1% Foreign Starch: None detected Gelatin: None detected Water Absorption: 75ml Acid-insoluble Ash: 0.5% Arsenic: 0.0003% Lead: 0.001% Heavy Metals (Pb): 0.004% Microbiological Analysis: Total Plate Count Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Falk, Bucca and Simmons, J. Bacteriology, 37: 121 (1939) 2. Hitchens and Leikind, J. Bacteriology, 37: 485 (1939) 3. Seip, In Fogg and Jones (ed), Proc. 8th Intl. Seaweed Symp. (1981) 4. United States Pharmacopeia, 8/14/12: Changed Lead: 0.001% -LP 04/21/11- Added Identification a,b, matter, starch, gelatin, absorption, ash. -JE 2/9/10: Changed Description: Gelidium based Drosophila agar is an inexpensive growth medium especially suited to the growth and nutrient requirements of Drosophila without the expense of bacteriological grades. This is a food grade agar which offers excellent clarity. -LP 5/15/09-remove: To Pass USP24/NF19 -add Exceed USP Specifications -DF 04/07/05-Updated to read similar: A0930, -05. -Changed Appearance: White to cream colored powder -Changed pH (1.5%): 6.0-7.5 -VV23: 1721,1897 (1990) |