Technical Data
A1025
Agarose High Gel Temp
50g
100g
Molecular Biology Storage: RTShipping: RT
High Gel Strength Agarose Combines very high gel strength with low EEO and is a good choice for high speed, high temperature pulsed field gel electrophoresis applications.

Applications:
Pulsed Field Electrophoresis
Immunoelectrophoresis
Blotting Assays (Southern, northern)
Analytical DNA Gels 1000bp
Preparative Electrophoresis
For use with all buffer systems

Recommended Usage (1X TAE):
Concentration Molecular Weight
0.4-2.0% 50-500kb

Storage and Stability: Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4C for 1 hour before use.

CAS Number:
9012-36-6
EEO: 0.14
Melting Point (1.5%): 85.5-88.5C
Gel Point (1.5%): 39.5-42.5C
Gel Strength (1.5%): 1200g/cm2
Clarity: 50 NTU
Sulfate: 0.2%
RNase: None Detected
DNase: None Detected

Quality Control: Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.

Functional and Molecular Biology Testing: To Pass Test
Comparative assay of different size DNA fragments
Southern Blot (125-23,000bp)
Background Fluorescence (TAE/TE, EtBr):
DNA Binding (TAE, EtBr)
Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ausubel, F.M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.