Technical Data
Aggrecan G1-IGD-G2 Domains (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, M
As the key component of the cartilage extracellular matrix, aggrecan hydrates the collagen network and provides cartilage with its properties of compressibility and elasticity. Maintenance of aggrecan content is therefore critical to the function of the tissue and aggrecan degradation is an important factor in the erosion of articular cartilage in arthritic diseases. The deduced amino acid sequence of human aggrecan core protein consists of 2415 residues and predicts a signal peptide and domains of G1, IGD, G2, KS, CS­1, CS­2 and G3 (3). Two globular domains, G1 and G2, comprise the N­terminus of the proteoglycan and also contain link domains. The third globular domain, G3, corresponds to the C­terminus. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains are between G2 and G3. With KS and CS attached to the 250kD core protein, aggrecan monomers exist as a 1000-2000kD molecule.

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml the detection limit is ~2ng/well
Western Blot: 1-2ug/ml the detection limit is ~5ng/lane under non-reducing conditions
Immunohistochemistry: 25ug/ml detects aggrecan in paraffin-embedded human cartilage tissue sections.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human aggrecan from conditioned media of transfected NSO cells. Dectection using A1059-53H.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2b9H46Affinity Purified
500ug-20°CBlue IceHumanMouse
~0.5mg/ml (after reconstitution)
Recombinant corresponding to aa20-675 from human Aggrecan G1-IGD-G2 Domains, expressed in NS0 cells (NP_037359).
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Recognizes human Aggrecan G1-IGD-G2 Domains.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.