Technical Data
A1059-53Q
Aggrecan (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen Identified by Monoclonal A0122, Cartilage Specific Proteoglycan Core Protein, Chondroitin Sulfate Proteoglycan Core Protein 1, CSPCP, CSPG1, CSPGCP, Large Aggregating Proteoglycan, MSK16, SEDK)
Description:
Aggrecan is a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS). Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
ELISA: Capture
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15J14Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human natural aggrecan (proteoglycan), purified from human articular cartilage
Purity:
Purified (> 95% SDS-PAGE)
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Recognizes natural human aggrecan. The epitopes recognized by this antibody are keratin sulfate (KS) residues.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Almqvist, K.F., et al., Ann. Rheum. Dis. 60(8): 781-790 (2001). 2. Guerne, P.A., et al., Matrix Biology 18: 253-260 (1999). 3. Wang, J., et al., Arthritis Rheum. 48(5): 1281-1291 (2003). 4. Wang, J., et al., Ann. Rheum. Dis. 63(1): 61-66 (2004). 5. Wang, J., et al., Osteoarthritis Cartilage 11(11): 801-809 (2003). 6. Wang, L., et al., Osteoarthritis Cartilage 9(1): 73-84 (2001). 7. Wang, L., et al., Clin. Exp. Rheumatol. 20(5): 669-676 (2002). 8. Wang, L., et al., Osteoarthritis Cartilage 9(5): 454-462 (2002). 9. Wang, L., et al., Osteoarthritis Cartilage 10(3): 188-198 (2002).