Technical Data
A1059-54M
Aggrecan FFGxx (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)
Description:
This fragment is rapidly released from the tissue when MMP catabolism of aggrecan occurs and has been identified in synovial fluid samples from patients with degenerative joint diseases.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,l8J210Supernatant
SizeStorageShippingSourceHost
1ml4°C (-20°C Glycerol)Blue IceMouse
Concentration:
Not determined
Immunogen:
Synthetic peptide: FFGVGGE..
Purity:
Tissue culture supernatant
Form
Supplied as a liquid in 0.2M Tris HCll, pH 7.4, 01-10% Fetal Calf Serum, 0.002% sodium azide.
Specificity:
Recognizes the N-terminal neoepitope sequence (FFGV....) generated at the ?MMP cleavage site? after MMP catabolism in the interglobular domain of aggrecan between amino acids ..PEN341 and 342FFG.. (Human sequence enumeration). Detects bands between approximately 60 and 150kD. Species Crossreactivity: Crossreacts with Human, Rat, bovine, Guinea pig, Horse and Pig.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002). Caterson B et al. Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biol 19:333-44 (2000).