Technical Data
A1059-62N
AGRP (Agouti-related Protein, AGRT, ART)
Description:
AGRP, secreted hypothalamic factors regulated by the metabolic state in mammals, is involved in energy homeostasis and plays an important role in the control of feeding behavior. Agouti protein and Agouti-related protein are paracrine-signaling molecules that normally regulate pigmentation and body weight. The mature human protein is a single polypeptide chain of 112aa residues consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. Circular dichroism studies indicated that the purified protein contains primarily random coil and beta-sheet secondary structure. The hypothalamic expression of AGRP is regulated by leptin, and overexpression of AGRP in transgenic animals results in obesity and diabetes.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:32,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CRKLGTAMNPCSRT, from the C-terminus of the protein sequence according to NP_001129.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes AGRP. Species sequence homology: chimpanzee, canine, human, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.