Technical Data
AGRP (Agouti-related Protein, AGRT, ART)
AGRP, secreted hypothalamic factors regulated by the metabolic state in mammals, is involved in energy homeostasis and plays an important role in the control of feeding behavior. Agouti protein and Agouti-related protein are paracrine-signaling molecules that normally regulate pigmentation and body weight. The mature human protein is a single polypeptide chain of 112aa residues consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. Circular dichroism studies indicated that the purified protein contains primarily random coil and beta-sheet secondary structure. The hypothalamic expression of AGRP is regulated by leptin, and overexpression of AGRP in transgenic animals results in obesity and diabetes.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:32,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to CRKLGTAMNPCSRT, from the C-terminus of the protein sequence according to NP_001129.1.
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Recognizes AGRP. Species sequence homology: chimpanzee, canine, human, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.