Technical Data
AHA1, Recombinant, Human (Activator of Hsp90 ATPase 1)
Molecular Biology Storage: -70CShipping: Dry Ice
The co-chaperone Aha1 (Activator of HSP90 ATPase-1) binds the middle domain of Hsp90 (aa272-627) and is proposed to function as an ATPase-activating protein that competes with p23 and other co-chaperones for Hsp90 binding.

Recombinant human, AHA1, full length, with C-terminal HIS tag, expressed in E. coli.

Molecular Weight: 38kD

Specific Activity:
The addition of AHA1 increased ATPase activity of hsp90-alpha from 0.18 to 0.94pmol/min/ug.

Assay conditions:
50ul reaction mix (100mM TrisHCl pH7.4, 20mM KCl, 6mM MgCl2, 200uM ATP, and 5ug Hsp90a) was incubated with Aha1 (0-10g) for 3 hrs at 37C. To stop the reaction, 100ul of the malachite green reagent was added to each well. Absorbance at 620nm was measured.

Suitable for use as ATPase activity assay for Hsp90-alpha. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Molecular Weight:
Source: E. coli
Purity: 90%
Concentration: As reported
Form: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Wang, X. et al., Cell 127 (4), 803815 (2006). 2. Lotz, G.P. et al., J. Biol. Chem. 278 (19), 1722817235 (2003).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.