Technical Data
A1059-70A
AHA1, Recombinant, Human (Activator of Hsp90 ATPase 1)
200ug
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Technical Data |
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A1059-70A |
AHA1, Recombinant, Human (Activator of Hsp90 ATPase 1) |
200ug |
| Molecular Biology | Storage: -70°CShipping: Dry Ice |
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The co-chaperone Aha1 (Activator of HSP90 ATPase-1) binds the middle domain of Hsp90 (aa272-627) and is proposed to function as an ATPase-activating protein that competes with p23 and other co-chaperones for Hsp90 binding. Source: Recombinant human, AHA1, full length, with C-terminal HIS tag, expressed in E. coli. Molecular Weight: 38kD Specific Activity: The addition of AHA1 increased ATPase activity of hsp90-alpha from 0.18 to 0.94pmol/min/ug. Assay conditions: 50ul reaction mix (100mM TrisHCl pH7.4, 20mM KCl, 6mM MgCl2, 200uM ATP, and 5ug Hsp90a) was incubated with Aha1 (0-10g) for 3 hrs at 37°C. To stop the reaction, 100ul of the malachite green reagent was added to each well. Absorbance at 620nm was measured. Applications: Suitable for use as ATPase activity assay for Hsp90-alpha. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. Molecular Weight: 38kD |
Source: E. coli Purity: 90% Concentration: As reported Form: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Wang, X. et al., Cell 127 (4), 803815 (2006). 2. Lotz, G.P. et al., J. Biol. Chem. 278 (19), 1722817235 (2003).
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