Technical Data
A1102-82
AKAP8 (A kinase (PRKA) anchor protein 8, A kinase anchor protein 8, AKAP95, DKFZp586B1222)
Description:
The A kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This antibody recognizes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:25,000-1:35,000
Western Blot: 1-3 ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Human Liver lysate.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
As reported
Immunogen:
Synthetic peptide DQGYGGYGAWSAG, representing the N-terminus of the human protein (residues 2-14)according to NP_005849. Molecular Weight: 96 kD. Localization: Nuclear. Associated with the nuclear matrix. Redistributed and detached from condensed chromati
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in tris-saline containing 0.02% sodium azide (pH7.3) with 0.5% BSA.
Specificity:
Recognizes human AKAP8. Species sequence homology: rat (91%), canine (99%) and mouse (99%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Eide T et al. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Exp Cell Res 238:305-16 (1998).