		Technical Data
A1102-83E	AKAP10 (A Kinase (PRKA) Anchor Protein 10, D-AKAP2, MGC9414, PRKA10, A-kinase Anchor Protein 10, Dual-specificity A-kinase Anchoring Protein 2, Mitochondrial A Kinase PPKA Anchor Protein 10, Protein Kinase A Anchoring Protein 10)

Description:
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 0.3-1 g/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to human AKAP10.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Specificity:

Recognizes human AKAP10 at ~74kD in Rat Brain lysates (calculated MW of 73.8kD according to human NP_009133.2 and according to Rat NP_001108078.1). Species Crossreactivity: Bovine, canine, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Burns-Hamuro LL, Barraclough DM, Taylor SS. Identification and functional analysis of dual-specific A kinase-anchoring protein-2. Methods Enzymol. 2004;390:354-74.