Technical Data
AKAP10 (A Kinase (PRKA) Anchor Protein 10, D-AKAP2, MGC9414, PRKA10, A-kinase Anchor Protein 10, Dual-specificity A-kinase Anchoring Protein 2, Mitochondrial A Kinase PPKA Anchor Protein 10, Protein Kinase A Anchoring Protein 10)
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 0.3-1 g/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
As reported
Synthetic peptide corresponding to human AKAP10.
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Recognizes human AKAP10 at ~74kD in Rat Brain lysates (calculated MW of 73.8kD according to human NP_009133.2 and according to Rat NP_001108078.1). Species Crossreactivity: Bovine, canine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Burns-Hamuro LL, Barraclough DM, Taylor SS. Identification and functional analysis of dual-specific A kinase-anchoring protein-2. Methods Enzymol. 2004;390:354-74.