Technical Data
AKT3, active, Recombinant, Human (RAC-gamma Serine/Threonine-protein Kinase, RAC-PK-gamma, Protein Kinase Akt-3, Protein Kinase B gamma, PKB gamma, PKBG, STK-2)
Molecular Biology Storage: -70CShipping: Dry Ice
Akt3 or Protein Kinase B g (PKB) is a serine/threonine kinase that is a member of the Akt family. In mammals, the Akt family comprises of three highly homologous members known as Akt1 (PKB), Akt2 (PKB), and Akt 3. Akt 3 like the other family members is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components. The activation mechanism remains to be fully characterized but occurs downstream of phosphoinositide 3-kinase (PI3K). PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt family members to the plasma membrane where they are phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) and phosphoinositide-dependent kinase-2 (PDK-2 possibly ILK). Akt 3 like the other family members phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis, and proliferation. Recent evidence indicates that Akt 3 is frequently overexpressed in many types of human cancers including breast and prostate. Although the mechanisms have not yet been fully characterized, increased expression and activation of Akt is believed to promote cell proliferation and survival thereby contributing to cancer progression.

Recombinant corresponding to full-length human Akt 3 containing N-terminal GST tag, expressed by baculovirus in Sf9 insect cells.

Molecular Weight:

Biological Activity:
206nmol phosphate incorporated into Akt substrate peptide per minute per mg protein at 30C for 15 minutes using a final concentration of 50uM ATP and total of 0.83uCi/ul P-32.

Specific Activity:

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight:
Source: Sf9 insect cells
Purity: ~92% (SDS-PAGE)
Concentration: As reported
Form: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.