Technical Data
Alarin (del/E3, Galanin-like Peptide, GALP)
Alarin is a hypothalamic neuropeptide which binds to the G-protein-coupled galanin receptors (GALR1, GALR2, and GALR3). Involved in a large number of putative physiological functions in CNS homeostatic processes, including the regulation of gonadotropin-releasing hormone secretion. Isoform 2 exhibits potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, a physiologic effects which does not appear to be mediated via GALR1 or GALR2 (By similarity). Cleavage of the signal peptide generates a peptide of 25 amino acids, termed alarin because of the N-terminal alanine and the C-terminal serine. Involved in ganglionic differentiation in neuroblastic tumor tissues.

Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200
Immunohistochemistry (formalin-fixed paraffin): 1:500 (PAP or ABC) 1 hour at RT. No pretreatment required. Recommended Counterstained Hematoxylin.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibody:
I1904-43: IgG, X-Adsorbed (Biotin) Pab GtxRb
I1904-40E: IgG, H&L (Biotin) Pab
I1904-46P: IgG, H&L (Biotin) GtxRb

Positive Control:
Mouse/Rat Brain Tissue

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceRatRabbit
Not determined
Synthetic peptide corresponding to rat Alarin.
Supplied as a lyophilized powder. Reconstitute with 100ul sterile dH2O.
Recognizes rat Alarin. Species crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
General References:
1. Ohtaki T., Kumano S., Ishibashi Y., Ogi K., Matsui H., Harada M., Kitada C., Kurokawa T., Onda H., Fujino M.; "Isolation and cDNA cloning of a novel galanin-like peptide (GALP) from porcine hypothalamus."; J. Biol. Chem. 274:37041-37045(1999).
2. Kofler B., Moritz K., Fenninger K.; "Differential splicing leads to the novel peptide alarin another member of the galanin-like peptide family."
3. Santic R., Fenninger K., Graf K., Schneider R., Hauser-Kronberger C., Schilling F.H., Kogner P., Ratschek M., Jones N., Sperl W., Kofler B.; "Gangliocytes in neuroblastic tumors express alarin, a novel peptide derived by differential splicing of the galanin-like peptide gene."; J. Mol. Neurosci. 29:145-152(2006).
4. Santic R., Schmidhuber S.M., Lang R., Rauch I., Voglas E., Eberhard N., Bauer J.W., Brain S.D., Kofler B.; "Alarin is a vasoactive peptide."; Proc. Natl. Acad. Sci. U.S.A. 104:10217-10222(2007).