Technical Data
A1334-70W
Aldo-Keto Reductase Family 1 Member B10 (AKR1B10, Aldose Reductase-like, Aldose Reductase-related Protein, ALDRLn, ARL 1, ARL-1, hARP, HIS, HSI, MGC14103, Small Intestine Reductase, SI Reductase)
Description:
A 316aa protein, AKR1B10 is a highly efficient retinal reductase constituting the first cytosolic NADP (H)-dependent retinal reductase described in humans and is characterized by the presence of a novel NADP binding motif. Due to its ability to convert glucose to sorbitol, AKR1B10 may have a role in development of secondary diabetic complications. It is abundantly expressed in adrenal gland, small intestine and colon, with lower levels in liver, thymus, prostate, testis, and skeletal muscle and its levels are up regulated in certain cancers such as hepatocellular carcinoma.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa60-73 (QEKIQEKAVKREDL) of human AKR1B10.
Purity:
Serum.
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human Aldo-keto reductase family 1 member B10. Species sequence homology: mouse, rat, chimpanzee, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Crosas,B., Hyndman,D.J., Gallego,O., Martras,S., Pares,X., Flynn,T.G. and Farres,J. Human aldose reductase and human small intestine aldose reductase are efficient retinal reductases: consequences for retinoid metabolism. Biochem. J. 373 (PT 3), 973-979 (2003). 2. Petrovic,M.G., Peterlin,B., Hawlina,M. and Petrovic,D. Aldose reductase (AC)n gene polymorphism and susceptibility to diabetic retinopathy in Type 2 diabetes in Caucasians. J. Diabetes Complicat. 19 (2), 70-73 (2005). 3. Cao, D et.al J. Biol. Chem. 273: 11429-11435 (1998) 4. Scuric, Z et.al Hepatology 27, 943-950 (1998).