Technical Data
A1379-47
Aminopeptidase O (AP-O, ONPEP)
Description:
Aminopeptidase O (AP-O; also C9orf3) is a 93kD (predicted), intracellular member of the M1 metalloprotease family of enzymes. It is expressed in heart, testis and pancreas where it apparently cleaves N-terminal Arg and Asn residues. Human AP-O is 819 amino acids in length. It contains one catalytic domain (aa 167-589) with a Zn-binding site (His479-His483) and a C-terminal ARM region (aa 706-819). At least three additional isoforms exist. One shows a deletion of aa 455-553; a second has an 11 aa substitution for the C-terminal 265 amino acids; and a third shows both a deletion of aa 455-553 and an 11 aa substitution for the C-terminal 46 amino acids.

Applications:
Suitable for use in Direct ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml. Detection limit for rhAminopeptidase O is ~0.3ng/well.
Western Blot: 0.1-0.2ug/ml. Detection limit for rhAminopeptidase O is ~5ng/lane under non-reducing and reducing conditions.
Immunoprecipitation: Used to precipitate rhAminopeptidase O from Sf21 lysates.
Immunohistochemistry: 3-10ug/ml. Antigen retrieval is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanGoat
Concentration:
~0.2mg/ml
Immunogen:
Purified Sf 21-derived rhAminopeptidase O isoform 1 (aa 1-819).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
Specificity:
Recognizes human Aminopeptidase O. Species sequence homology: mouse, 76%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.