Technical Data
A1381-50
7-Amino-4-trifluoromethylcoumarin (AFC)
100mg
Molecular Biology Storage: -20CShipping: Blue Ice
Solubility:
Soluble in methanol or 25mg/ml DMSO.

Applications:
The free form of AFC can be used as a fluorescent marker and standard in fluororometric caspase assays.

Protocol:
Prepare a 1mM solutionof AFC in methanol or DMSO and then dilute it into aqueous buffer at different concentrations within the 0-4uM range. Read 100ul/sample.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with methanol or DMSO. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Formula:
C10H6NO2F3

Molecular Weight:
229.2
Purity: > 99% (TLC)
Form: Supplied as a yellow powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Fletcher, A., et al., Appl. Phys. 45: 295-299 (1977). 2. Bissel, E.R., et al., J. Org. Chem. 45: 2283-2289 (1980).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.