Technical Data
A1381-50
7-Amino-4-trifluoromethylcoumarin (AFC)
100mg
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Technical Data |
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A1381-50 |
7-Amino-4-trifluoromethylcoumarin (AFC) |
100mg |
| Molecular Biology | Storage: -20°CShipping: Blue Ice |
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Solubility: Soluble in methanol or 25mg/ml DMSO. Applications: The free form of AFC can be used as a fluorescent marker and standard in fluororometric caspase assays. Protocol: Prepare a 1mM solutionof AFC in methanol or DMSO and then dilute it into aqueous buffer at different concentrations within the 0-4uM range. Read 100ul/sample. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with methanol or DMSO. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Formula: C10H6NO2F3 Molecular Weight: 229.2 |
Purity: > 99% (TLC) Form: Supplied as a yellow powder. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Fletcher, A., et al., Appl. Phys. 45: 295-299 (1977). 2. Bissel, E.R., et al., J. Org. Chem. 45: 2283-2289 (1980).
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